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Test Code LAB1242 Hepatitis B Core Total Antibodies

Important Note

HBc Total assay is an in vitro diagnostic test for the qualitative determination of total antibodies to the core antigen of the hepatitis B virus (HBc Total) in human neonatal, pediatric, and adult serum or plasma. This assay can be used as an aid in the diagnosis of individuals with acute or chronic hepatitis B virus (HBV) infection and in the determination of the clinical status of HBV infected individuals in conjunction with other HBV serological markers for the laboratory diagnosis of HBV disease associated with HBV infection. This assay can also be used as an aid in the differential diagnosis in individuals displaying signs and symptoms of hepatitis in whom etiology is unknown.

Methodology

The ADVIA Centaur HBc Total assay is a two wash antigen sandwich immunoassay in which antigens are bridged by antibody present in the patient sample. The Solid Phase contains a preformed complex of streptavidin coated microparticles and biotinylated recombinant HBc antigen and is used to capture anti-HBc in the patient sample. The Lite Reagent contains recombinant HBc antigen labeled with acridinium ester and is used to detect anti-HBc in the sample. Solid Phase and Chaotrope Reagent are added to the sample, followed by Ancillary Reagent and Lite Reagent. Antibody-antigen complexes will form if anti-HBc antibodies (IgM and IgG) are present in the sample.

Performing Laboratory

RCH

Specimen Requirements

Preferred: Serum

Container/tube: Plain, red-top or gold, serum gel tube

Acceptable: Plasma (heparinized)

Container: Lt Green/Mint top (lithium heparin) tube

Specimen Volume:   1.0 mL (minimum volume 0.1 mL)

Collection Instructions: Forward promptly

Specimen Stability

Ambient: 12 hours

Refrigerated: 3 days

Frozen: Indefinite (2 freeze/thaw cycles)

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding

86704