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Test Code TBIDS Culture Referred for Identification, Mycobacterium and Nocardia with Antimicrobial Susceptibility Testing, Varies

Infectious


Ordering Guidance


This test is intended for Mycobacterium species, Nocardia species, or other aerobic actinomycete genera.



Shipping Instructions


1. See Infectious Specimen Shipping Guidelines for shipping information.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.



Necessary Information


1. Specimen source is required.

2. Isolate description is required: Gram stain reaction, morphology, tests performed.



Specimen Required


Specimen Type: Organism in pure culture

Supplies: Infectious Container, Large (T146)

Container/Tube: Middlebrook (7H10 or 7H11) or Lowenstein-Jensen medium slant or in broth (eg, Mycobacteria Growth Indicator Tube [7H9] broth)

Specimen Volume: Isolate

Collection Instructions: Organism must be in pure culture, actively growing. Do not submit mixed cultures.


Useful For

Rapid identification to the species level and susceptibility testing for Mycobacterium species, Nocardia species, and other aerobic actinomycete genera and species from pure culture isolates

Testing Algorithm

When this test is ordered, reflex antimicrobial susceptibility testing will be performed at an additional charge. All mycobacteria and Nocardia (including aerobic actinomycetes) submitted will be identified and billed as appropriate.

 

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF MS) and/or 16S rDNA sequencing is used for identification, when applicable, for slowly and rapidly growing Mycobacterium species and aerobic actinomycetes.

 

Mycobacterium tuberculosis complex rapid polymerase chain reaction (PCR) is used to rule out M tuberculosis complex from all broth specimens received with sufficient volume. Testing on solid growth is determined based on growth rate, colony morphology, or specific request by clients.

 

The M tuberculosis complex will be further identified to the species level upon request using rapid PCR testing.

 

Minimum inhibitory concentration (MIC) determination by either the microtiter broth dilution method or critical concentration testing by broth dilution will be automatically performed as appropriate after species identification.

 

For more information see Culture Referred for Identification and Susceptibility for Mycobacterium and Nocardia Algorithm.

Method Name

16S rDNA Sequencing/Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI TOF MS)/Rapid Polymerase Chain Reaction (PCR)

Reporting Name

Mycobacteria Culture Refer ID+Susc

Specimen Type

Varies

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Ambient (preferred)
  Refrigerated 

Reference Values

Not applicable

Day(s) Performed

Monday through Sunday

Report Available

60 to 70 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

Culture Referred for Identification, Mycobacterium

87118-Identificaiton of mycobacteria

87158-Identification of mycobacteria by other methods (if appropriate)

87118 -Id MALDI-TOF Mass Spec AFB (if appropriate)

87153-Mycobacteria Identification by Sequencing (if appropriate)

87150-Id, Mtb Speciation, PCR (if appropriate)

87186-Susceptibility Rapid Grower (if appropriate)

87186-Susceptibility Slow Grower (if appropriate)

87186-Susceptibility Nocardia species (if appropriate)

87188 x 3-Antimicrobial Susceptibility, Mycobacterium tuberculosis Complex, Broth Method (if appropriate)

87186-Suscetpbility, Mtb Cx, 2nd Line (if appropriate)

87188-Susceptibility, Mycobacterium tuberculosis Complex, Pyrazinamide (if appropriate)

87153-Mtb PZA Confirmation, pncA Sequencing (if appropriate)

87150- Id, MTB complex Rapid PCR (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TBIDS Mycobacteria Culture Refer ID+Susc 543-9

 

Result ID Test Result Name Result LOINC Value
TBIDS Mycobacteria Culture Refer ID+Susc In Process

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.