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HelpTest Code VLTBX Volatile Screen, Chain of Custody, Blood
Useful For
Detection and quantitation of acetone, methanol, isopropanol, and ethanol in whole blood
Quantification of the concentration of ethanol in blood that correlates with the degree of intoxication
Evaluation of toxicity to the measured volatile substances
This test is not intended for use in employment-related testing.
Providing chain-of-custody for when the results of testing could be used in a court of law. Its purpose is to protect the rights of the individual contributing the specimen by demonstrating that it was always under the control of personnel involved with testing the specimen; this control implies that the opportunity for specimen tampering would be limited.
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| COCH | Chain of Custody Processing | No | Yes |
Testing Algorithm
This test includes analysis of methanol, ethanol, isopropanol, and acetone.
Reporting Name
Volatile Screen, CoC, BSpecimen Type
Whole bloodSpecimen Required
Supplies: Chain-of-Custody Kit (T282)
Container/Tube: Chain-of-Custody Kit containing the specimen container seals and documentation required.
Preferred: Gray top (potassium oxalate/sodium fluoride)
Acceptable: Lavender top (EDTA) or green top (sodium heparin)
Specimen Volume: 2 mL
Collection Instructions:
1. Do not use alcohol to clean arm. Use alternative such as Betadine to cleanse arm before collecting any specimen for volatile testing.
2. Specimen must be sent in original tube. Collect specimen, seal, and submit with the associated documentation to satisfy the legal requirements for chain of custody testing.
Specimen Minimum Volume
0.5 mL or amount to fill 1 tube
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole blood | Refrigerated (preferred) | 14 days | |
| Frozen | 28 days | ||
| Ambient | 24 hours |
Reference Values
Methanol:
Not detected (Positive results are quantitated.)
Toxic concentration: ≥10 mg/dL
Ethanol:
Not detected (Positive results are quantitated.)
Toxic concentration: ≥400 mg/dL
Isopropanol:
Not detected (Positive results are quantitated.)
Toxic concentration: ≥10 mg/dL
Acetone:
Not detected (Positive results are quantitated.)
Toxic concentration: ≥10 mg/dL
Day(s) Performed
Monday through Saturday
Report Available
1 to 2 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
80320
G0480 (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| VLTBX | Volatile Screen, CoC, B | 41266-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 36241 | Volatile Scrn, B | 41266-8 |
| 36242 | Methanol, B | 9334-4 |
| 36243 | Ethanol, B | 5640-8 |
| 36244 | Acetone, B | 9425-0 |
| 36245 | Isopropanol, B | 5667-1 |
| 36246 | Chain of Custody | 77202-0 |
Method Name
Headspace Gas Chromatography Flame Ionization Detector (HSGC-FID)
Forms
Chain of Custody Request is included in the Chain-of-Custody Kit (T282)